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anti tfrc rabbit polyclonal antibody  (Proteintech)


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    Proteintech anti tfrc rabbit polyclonal antibody
    Adipsin-enriched pericardial-AT exosomes regulate iron homeostasis and alleviate lipid oxidative stress in vivo . (A,B) Non-heme iron levels in the serum and the peri-infarct region of the cardiac tissue were assessed in MI mouse after treatment with Adipsin-transgenic or non-transgenic exosomes. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (C) The levels of malondialdehyde (MDA) in cardiac tissue were measured in the peri-infarct area. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (D) Perls’ Prussian Blue-stained heart slices. (E–I) <t>TFRC,</t> FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.
    Anti Tfrc Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tfrc+rabbit+polyclonal+antibody/CD71+Antibody/pmc09510661-134-26-30
    Average 96 stars, based on 285 article reviews
    anti tfrc rabbit polyclonal antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis"

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis

    Journal: Frontiers in Cardiovascular Medicine

    doi: 10.3389/fcvm.2022.1003282

    Adipsin-enriched pericardial-AT exosomes regulate iron homeostasis and alleviate lipid oxidative stress in vivo . (A,B) Non-heme iron levels in the serum and the peri-infarct region of the cardiac tissue were assessed in MI mouse after treatment with Adipsin-transgenic or non-transgenic exosomes. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (C) The levels of malondialdehyde (MDA) in cardiac tissue were measured in the peri-infarct area. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (D) Perls’ Prussian Blue-stained heart slices. (E–I) TFRC, FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.
    Figure Legend Snippet: Adipsin-enriched pericardial-AT exosomes regulate iron homeostasis and alleviate lipid oxidative stress in vivo . (A,B) Non-heme iron levels in the serum and the peri-infarct region of the cardiac tissue were assessed in MI mouse after treatment with Adipsin-transgenic or non-transgenic exosomes. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (C) The levels of malondialdehyde (MDA) in cardiac tissue were measured in the peri-infarct area. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (D) Perls’ Prussian Blue-stained heart slices. (E–I) TFRC, FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.

    Techniques Used: In Vivo, Transgenic Assay, Staining, Western Blot

    Irp2 is essential to Adipsin-offered cardioprotection. (A) Flowchart of the study design. Myocardial infarction surgery was performed 14 days after injection of AAV9 (Scramble or IRP2-shRNA) at the area of the myocardium. Following that, MI mice were injected four times with exosomes isolated from Adipsin-Tg or NTg mice’s pericardial adipose tissues. Cardiac function was assessed 28 days after MI surgery. Each group n = 6. (B,C) Representative echocardiographic images and left ventricular ejection fraction (LVEF) analysis. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (D) Non-heme iron levels were measured in the peri-infarct region of heart. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (E,F) Quantification of fibrotic area and representative images of Masson’s trichrome staining of the transverse planes post myocardial infarction. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (G) Malondialdehyde (MDA) levels in the peri-infarct region of heart. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (H–L) TFRC, FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.
    Figure Legend Snippet: Irp2 is essential to Adipsin-offered cardioprotection. (A) Flowchart of the study design. Myocardial infarction surgery was performed 14 days after injection of AAV9 (Scramble or IRP2-shRNA) at the area of the myocardium. Following that, MI mice were injected four times with exosomes isolated from Adipsin-Tg or NTg mice’s pericardial adipose tissues. Cardiac function was assessed 28 days after MI surgery. Each group n = 6. (B,C) Representative echocardiographic images and left ventricular ejection fraction (LVEF) analysis. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (D) Non-heme iron levels were measured in the peri-infarct region of heart. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (E,F) Quantification of fibrotic area and representative images of Masson’s trichrome staining of the transverse planes post myocardial infarction. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (G) Malondialdehyde (MDA) levels in the peri-infarct region of heart. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (H–L) TFRC, FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.

    Techniques Used: Injection, shRNA, Isolation, Staining, Western Blot

    Related Articles

    In Vivo:

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), anti-transferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis.
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), antitransferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Transgenic Assay:

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), anti-transferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis.
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), antitransferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Staining:

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), anti-transferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis.
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), antitransferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Western Blot:

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), anti-transferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis.
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), antitransferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Injection:

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), anti-transferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis.
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), antitransferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    shRNA:

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), anti-transferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis.
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), antitransferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Isolation:

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), anti-transferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis.
    Article Snippet: WB densitometry was quantified using the Image Lab system.WB densitometry was quantified using the Image Lab system.. Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), antitransferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).



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    Adipsin-enriched pericardial-AT exosomes regulate iron homeostasis and alleviate lipid oxidative stress in vivo . (A,B) Non-heme iron levels in the serum and the peri-infarct region of the cardiac tissue were assessed in MI mouse after treatment with Adipsin-transgenic or non-transgenic exosomes. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (C) The levels of malondialdehyde (MDA) in cardiac tissue were measured in the peri-infarct area. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (D) Perls’ Prussian Blue-stained heart slices. (E–I) <t>TFRC,</t> FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.
    Rabbit Polyclonal Antibody For Tfrc, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit polyclonal anti transferrin receptor c  (Sino Biological)
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    Adipsin-enriched pericardial-AT exosomes regulate iron homeostasis and alleviate lipid oxidative stress in vivo . (A,B) Non-heme iron levels in the serum and the peri-infarct region of the cardiac tissue were assessed in MI mouse after treatment with Adipsin-transgenic or non-transgenic exosomes. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (C) The levels of malondialdehyde (MDA) in cardiac tissue were measured in the peri-infarct area. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (D) Perls’ Prussian Blue-stained heart slices. (E–I) <t>TFRC,</t> FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.
    Rabbit Polyclonal Anti Transferrin Receptor C, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Adipsin-enriched pericardial-AT exosomes regulate iron homeostasis and alleviate lipid oxidative stress in vivo . (A,B) Non-heme iron levels in the serum and the peri-infarct region of the cardiac tissue were assessed in MI mouse after treatment with Adipsin-transgenic or non-transgenic exosomes. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (C) The levels of malondialdehyde (MDA) in cardiac tissue were measured in the peri-infarct area. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (D) Perls’ Prussian Blue-stained heart slices. (E–I) TFRC, FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis

    doi: 10.3389/fcvm.2022.1003282

    Figure Lengend Snippet: Adipsin-enriched pericardial-AT exosomes regulate iron homeostasis and alleviate lipid oxidative stress in vivo . (A,B) Non-heme iron levels in the serum and the peri-infarct region of the cardiac tissue were assessed in MI mouse after treatment with Adipsin-transgenic or non-transgenic exosomes. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (C) The levels of malondialdehyde (MDA) in cardiac tissue were measured in the peri-infarct area. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (D) Perls’ Prussian Blue-stained heart slices. (E–I) TFRC, FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6, * p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg, I p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg, § p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.

    Article Snippet: Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), anti-transferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Techniques: In Vivo, Transgenic Assay, Staining, Western Blot

    Irp2 is essential to Adipsin-offered cardioprotection. (A) Flowchart of the study design. Myocardial infarction surgery was performed 14 days after injection of AAV9 (Scramble or IRP2-shRNA) at the area of the myocardium. Following that, MI mice were injected four times with exosomes isolated from Adipsin-Tg or NTg mice’s pericardial adipose tissues. Cardiac function was assessed 28 days after MI surgery. Each group n = 6. (B,C) Representative echocardiographic images and left ventricular ejection fraction (LVEF) analysis. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (D) Non-heme iron levels were measured in the peri-infarct region of heart. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (E,F) Quantification of fibrotic area and representative images of Masson’s trichrome staining of the transverse planes post myocardial infarction. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (G) Malondialdehyde (MDA) levels in the peri-infarct region of heart. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (H–L) TFRC, FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis

    doi: 10.3389/fcvm.2022.1003282

    Figure Lengend Snippet: Irp2 is essential to Adipsin-offered cardioprotection. (A) Flowchart of the study design. Myocardial infarction surgery was performed 14 days after injection of AAV9 (Scramble or IRP2-shRNA) at the area of the myocardium. Following that, MI mice were injected four times with exosomes isolated from Adipsin-Tg or NTg mice’s pericardial adipose tissues. Cardiac function was assessed 28 days after MI surgery. Each group n = 6. (B,C) Representative echocardiographic images and left ventricular ejection fraction (LVEF) analysis. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (D) Non-heme iron levels were measured in the peri-infarct region of heart. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (E,F) Quantification of fibrotic area and representative images of Masson’s trichrome staining of the transverse planes post myocardial infarction. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (G) Malondialdehyde (MDA) levels in the peri-infarct region of heart. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (H–L) TFRC, FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6. * p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.

    Article Snippet: Antibodies used included anti-Adipsin rabbit monoclonal antibody (Abcam, ab213177, 1/1,000), anti-transferrin rabbit monoclonal antibody (Abcam, ab109503, 1/8,000), HRP anti-beta actin mouse monoclonal antibody (Abcam, ab20272, 1/5,000), anti-TFRC rabbit polyclonal antibody (Proteintech, 10084-2-AP, 1/2,000), anti-ferritin heavy chain rabbit monoclonal antibody (Abcam, ab183781, 1/1,000), anti-COX2 rabbit polyclonal antibody (ABclonal, A1253, 1/3,000), anti-GPX4 rabbit monoclonal antibody (ABclonal, A11243, 1/1,000), anti-GAPDH rabbit polyclonal antibody (Proteintech, 10494-1-AP, 1/8,000).

    Techniques: Injection, shRNA, Isolation, Staining, Western Blot